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Author: Daniel Marrama

PEPMatch is a high-performance peptide search tool for finding short peptide sequences within a reference proteome. Powered by a Rust engine with Python bindings, it delivers sub-second search times across entire proteomes while maintaining a simple Python API.

Key Features

• Blazing Fast: Rust-powered search engine with automatic multi-core parallelization via Rayon. Search thousands of peptides against the entire human proteome in seconds.
• Unified Index Format: Single .pepidx binary format stores sequences, metadata, and k-mer index in one memory-mapped file. Preprocess once, search repeatedly.
• Versatile Searching: Exact matches, mismatch-tolerant searches, best match mode, and discontinuous epitope support.
• Simple API: Two classes — Preprocessor and Matcher — handle everything.
• Flexible I/O: Accepts queries from FASTA files, text files, or Python lists. Outputs to CSV, TSV, XLSX, JSON, or Polars DataFrame.

Requirements

• Python 3.10+
• Polars
• Biopython

Installation

pip install pepmatch

Quick Start

from pepmatch import Preprocessor, Matcher

# Preprocess a proteome (one-time step)
Preprocessor('human.fasta').preprocess(k=5)

# Search for exact matches
df = Matcher(
  query=['YLLDLHSYL', 'GLCTLVAML', 'FAKEPEPTIDE'],
  proteome_file='human.fasta',
  max_mismatches=0,
  k=5
).match()

print(df)

Preprocessing

Preprocessing builds a .pepidx index from your proteome FASTA file. This only needs to be done once per proteome and k-mer size. If a .pepidx file doesn't exist when you search, Matcher will create it automatically.

from pepmatch import Preprocessor

Preprocessor('human.fasta').preprocess(k=5)

CLI:

pepmatch-preprocess -p human.fasta -k 5

Flags

• -p, --proteome (Required): Path to the proteome FASTA file.
• -k, --kmer_size (Required): The k-mer size for indexing.
• -n, --proteome_name: Custom name for the proteome.
• -P, --preprocessed_files_path: Directory to save preprocessed files.

Matching

Exact Matching

from pepmatch import Matcher

df = Matcher(
  query='peptides.fasta',
  proteome_file='human.fasta',
  max_mismatches=0,
  k=5
).match()

Mismatch Searching

df = Matcher(
  query='neoepitopes.fasta',
  proteome_file='human.fasta',
  max_mismatches=3,
  k=3
).match()

Best Match

Automatically finds the optimal match for each peptide by trying different k-mer sizes and mismatch thresholds. No manual preprocessing required.

df = Matcher(
  query='peptides.fasta',
  proteome_file='human.fasta',
  best_match=True
).match()

Discontinuous Epitope Searching

Search for non-contiguous residues defined by their positions.

df = Matcher(
  query=[
    "R377, Q408, Q432, H433, F436",
    "S2760, V2763, E2773, D2805, T2819"
  ],
  proteome_file='sars-cov-2.fasta',
  max_mismatches=1
).match()

Mixed Queries

Linear peptides and discontinuous epitopes can be searched together.

df = Matcher(
  query=[
    'YLLDLHSYL',
    'R377, Q408, Q432, H433, F436',
    'GLCTLVAML',
  ],
  proteome_file='sars-cov-2.fasta',
  max_mismatches=0
).match()

Query Input Formats

• Python list: ['YLLDLHSYL', 'GLCTLVAML']
• FASTA file: .fasta, .fas, .fa, .fna, .ffn, .faa, .mpfa, .frn
• Text file: .txt with one peptide per line

CLI:

pepmatch-match -q peptides.fasta -p human.fasta -m 0 -k 5

Flags

• -q, --query (Required): Path to the query file.
• -p, --proteome_file (Required): Path to the proteome FASTA file.
• -m, --max_mismatches: Maximum mismatches allowed (default: 0).
• -k, --kmer_size: K-mer size (default: 5).
• -P, --preprocessed_files_path: Directory containing preprocessed files.
• -b, --best_match: Enable best match mode.
• -f, --output_format: Output format — csv, tsv, xlsx, json (default: csv).
• -o, --output_name: Output file name (without extension).
• -v, --sequence_version: Disable sequence versioning on protein IDs.

Output Formats

• dataframe (default for API): Returns a Polars DataFrame.
• csv (default for CLI): CSV file.
• tsv: Tab-separated file.
• xlsx: Excel file.
• json: JSON file.

Performance

Benchmarked searching ~2,000 peptides against the human proteome (~200,000 proteins):

Mode	Time
Exact match (k=5)	~0.06s
1 mismatch (k=3)	~1.5s
2 mismatches (k=3)	~1.9s
3 mismatches (k=3)	~3.7s

Citation

If you use PEPMatch in your research, please cite:

Marrama D, Chronister WD, Westernberg L, et al. PEPMatch: a tool to identify short peptide sequence matches in large sets of proteins. BMC Bioinformatics. 2023;24(1):485. Published 2023 Dec 18. doi:10.1186/s12859-023-05606-4